High-resolution optical studies on C-phycocyanin via photochemical hole burning
نویسندگان
چکیده
منابع مشابه
Photochemical Hole-burning in Photosynthetic Reaction Centers
The change in absorbance (hole spectrum) of the primary electron donor (P870) in bacterial photosynthetic reaction centers has been studied at 1.5-2.1 K following narrow-band excitation at several wavelengths within the P870 absorption band. The hole width is very large, suggesting a homogeneous linewidth on the order of 200-300 cm-‘. Possible interpretations of this highly unusual result, incl...
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Photochemical hole burning has been observed at 1.5 K in bacteriorhodopsin (bR) and bovine rhodopsin at wavelengths spanning their lowest energy electronic absorption bands. Hole widths were -2500 cm-' full width at half-maximum (fwhm) in bacteriorhodopsin and -2400 cm-' fwhm in rhodopsin. The positions of the hole minima were insensitive to bum wavelength, indicating that minimal site selectio...
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A novel approach for interferometric analysis of ultrafast transient processes as short as 10(-13)-10(-14) sec is suggested. The method of holographic storage of ultrafast time-space images in spectrally selective hole-burning materials is used. It is shown that differential interferometric comparison of time-space images, which are permanently stored, provides an effective means to resolve ult...
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The kinetics of thermal denaturation of a biliprotein, C-phycocyanin (C-PC) isolated from Spirulina platensis were studied at different pH values, ranging from 4.0 to 8.0. The denaturation of C-PC follows the first order kinetics and rate constant at pH 5.0 and temperature 55 degrees C is found to be 4.37 x 10(-5) s(-1), which increases to 5.46 x 10(-1) s(-1) at pH 7.0. The denaturation rate is...
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Bile Pigments, Protein Interaction, Conformation, Denaturation The chromophore-protein interactions of C-phycocyanin (C-PC) from Spirulina platensis have been studied by following the partial and complete denaturation with UV-Vis spectroscopy. From comparison with published MO calculations, an elongated conformation of the chromophore is sug gested for native C-PC, a cyclic one for denatured C...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 1981
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja00395a006